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dapi counterstaining solution  (Vector Laboratories)


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    Vector Laboratories dapi counterstaining solution
    Dapi Counterstaining Solution, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 98/100, based on 21320 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dapi counterstaining solution/product/Vector Laboratories
    Average 98 stars, based on 21320 article reviews
    dapi counterstaining solution - by Bioz Stars, 2026-05
    98/100 stars

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    Activation of Wnt/β-catenin signaling enhances STB differentiation. (A) A scheme of the culture conditions. Undifferentiated macTSCs were cultured with [CHIR(+)] or without [CHIR(−)] CHIR99021 for 3 days. Some cells were induced to differentiate in the medium containing dbcAMP [CHIR(−) cAMP and CHIR(+) cAMP] for another 6 days. (B) The relative expression level of a vCTB marker, TEAD4 . (C) Phase contrast images of cells on day 9. Multinucleated STB is outlined by a red line. Scale bar = 100 μm. (D, G) The relative expression levels of STB (D) and EVT (G) markers. For C, D, and G, the mean values (±SD) of technical triplicates of biological duplicates, normalized by the expression of ACTB , are shown relative to those in CHIR(−) (arbitrarily set as 1). Different letters represent significant differences ( p < 0.05). (E) Detection of multinucleated STBs by immunofluorescence for E-cadherin, CG, and <t>DAPI</t> staining. Scale bar = 100 μm. (F) The mean intensity ± SD (%) of STB nuclei calculated from four randomly chosen fields of biological duplicates. Different letters represent significant differences ( p < 0.05). Note that error bars got larger as the STB ratio increased, likely because the larger the STBs formed, the sparser the distribution of STBs became so that some of the randomly selected fields contained very few STBs. (H) Detection of gelatinase activities of MMP2 and MMP9 by gelatin zymography.
    Counterstaining With Dapi, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Activation of Wnt/β-catenin signaling enhances STB differentiation. (A) A scheme of the culture conditions. Undifferentiated macTSCs were cultured with [CHIR(+)] or without [CHIR(−)] CHIR99021 for 3 days. Some cells were induced to differentiate in the medium containing dbcAMP [CHIR(−) cAMP and CHIR(+) cAMP] for another 6 days. (B) The relative expression level of a vCTB marker, TEAD4 . (C) Phase contrast images of cells on day 9. Multinucleated STB is outlined by a red line. Scale bar = 100 μm. (D, G) The relative expression levels of STB (D) and EVT (G) markers. For C, D, and G, the mean values (±SD) of technical triplicates of biological duplicates, normalized by the expression of ACTB , are shown relative to those in CHIR(−) (arbitrarily set as 1). Different letters represent significant differences ( p < 0.05). (E) Detection of multinucleated STBs by immunofluorescence for E-cadherin, CG, and <t>DAPI</t> staining. Scale bar = 100 μm. (F) The mean intensity ± SD (%) of STB nuclei calculated from four randomly chosen fields of biological duplicates. Different letters represent significant differences ( p < 0.05). Note that error bars got larger as the STB ratio increased, likely because the larger the STBs formed, the sparser the distribution of STBs became so that some of the randomly selected fields contained very few STBs. (H) Detection of gelatinase activities of MMP2 and MMP9 by gelatin zymography.
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    Activation of Wnt/β-catenin signaling enhances STB differentiation. (A) A scheme of the culture conditions. Undifferentiated macTSCs were cultured with [CHIR(+)] or without [CHIR(−)] CHIR99021 for 3 days. Some cells were induced to differentiate in the medium containing dbcAMP [CHIR(−) cAMP and CHIR(+) cAMP] for another 6 days. (B) The relative expression level of a vCTB marker, TEAD4 . (C) Phase contrast images of cells on day 9. Multinucleated STB is outlined by a red line. Scale bar = 100 μm. (D, G) The relative expression levels of STB (D) and EVT (G) markers. For C, D, and G, the mean values (±SD) of technical triplicates of biological duplicates, normalized by the expression of ACTB , are shown relative to those in CHIR(−) (arbitrarily set as 1). Different letters represent significant differences ( p < 0.05). (E) Detection of multinucleated STBs by immunofluorescence for E-cadherin, CG, and <t>DAPI</t> staining. Scale bar = 100 μm. (F) The mean intensity ± SD (%) of STB nuclei calculated from four randomly chosen fields of biological duplicates. Different letters represent significant differences ( p < 0.05). Note that error bars got larger as the STB ratio increased, likely because the larger the STBs formed, the sparser the distribution of STBs became so that some of the randomly selected fields contained very few STBs. (H) Detection of gelatinase activities of MMP2 and MMP9 by gelatin zymography.
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    dapi-based mounting solution dapi i counterstain 1000 ng/ml  (Abbott Laboratories)
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    Activation of Wnt/β-catenin signaling enhances STB differentiation. (A) A scheme of the culture conditions. Undifferentiated macTSCs were cultured with [CHIR(+)] or without [CHIR(−)] CHIR99021 for 3 days. Some cells were induced to differentiate in the medium containing dbcAMP [CHIR(−) cAMP and CHIR(+) cAMP] for another 6 days. (B) The relative expression level of a vCTB marker, TEAD4 . (C) Phase contrast images of cells on day 9. Multinucleated STB is outlined by a red line. Scale bar = 100 μm. (D, G) The relative expression levels of STB (D) and EVT (G) markers. For C, D, and G, the mean values (±SD) of technical triplicates of biological duplicates, normalized by the expression of ACTB , are shown relative to those in CHIR(−) (arbitrarily set as 1). Different letters represent significant differences ( p < 0.05). (E) Detection of multinucleated STBs by immunofluorescence for E-cadherin, CG, and <t>DAPI</t> staining. Scale bar = 100 μm. (F) The mean intensity ± SD (%) of STB nuclei calculated from four randomly chosen fields of biological duplicates. Different letters represent significant differences ( p < 0.05). Note that error bars got larger as the STB ratio increased, likely because the larger the STBs formed, the sparser the distribution of STBs became so that some of the randomly selected fields contained very few STBs. (H) Detection of gelatinase activities of MMP2 and MMP9 by gelatin zymography.
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    Activation of Wnt/β-catenin signaling enhances STB differentiation. (A) A scheme of the culture conditions. Undifferentiated macTSCs were cultured with [CHIR(+)] or without [CHIR(−)] CHIR99021 for 3 days. Some cells were induced to differentiate in the medium containing dbcAMP [CHIR(−) cAMP and CHIR(+) cAMP] for another 6 days. (B) The relative expression level of a vCTB marker, TEAD4 . (C) Phase contrast images of cells on day 9. Multinucleated STB is outlined by a red line. Scale bar = 100 μm. (D, G) The relative expression levels of STB (D) and EVT (G) markers. For C, D, and G, the mean values (±SD) of technical triplicates of biological duplicates, normalized by the expression of ACTB , are shown relative to those in CHIR(−) (arbitrarily set as 1). Different letters represent significant differences ( p < 0.05). (E) Detection of multinucleated STBs by immunofluorescence for E-cadherin, CG, and <t>DAPI</t> staining. Scale bar = 100 μm. (F) The mean intensity ± SD (%) of STB nuclei calculated from four randomly chosen fields of biological duplicates. Different letters represent significant differences ( p < 0.05). Note that error bars got larger as the STB ratio increased, likely because the larger the STBs formed, the sparser the distribution of STBs became so that some of the randomly selected fields contained very few STBs. (H) Detection of gelatinase activities of MMP2 and MMP9 by gelatin zymography.
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    counterstain solution  (Vector Laboratories)
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    Activation of Wnt/β-catenin signaling enhances STB differentiation. (A) A scheme of the culture conditions. Undifferentiated macTSCs were cultured with [CHIR(+)] or without [CHIR(−)] CHIR99021 for 3 days. Some cells were induced to differentiate in the medium containing dbcAMP [CHIR(−) cAMP and CHIR(+) cAMP] for another 6 days. (B) The relative expression level of a vCTB marker, TEAD4 . (C) Phase contrast images of cells on day 9. Multinucleated STB is outlined by a red line. Scale bar = 100 μm. (D, G) The relative expression levels of STB (D) and EVT (G) markers. For C, D, and G, the mean values (±SD) of technical triplicates of biological duplicates, normalized by the expression of ACTB , are shown relative to those in CHIR(−) (arbitrarily set as 1). Different letters represent significant differences ( p < 0.05). (E) Detection of multinucleated STBs by immunofluorescence for E-cadherin, CG, and <t>DAPI</t> staining. Scale bar = 100 μm. (F) The mean intensity ± SD (%) of STB nuclei calculated from four randomly chosen fields of biological duplicates. Different letters represent significant differences ( p < 0.05). Note that error bars got larger as the STB ratio increased, likely because the larger the STBs formed, the sparser the distribution of STBs became so that some of the randomly selected fields contained very few STBs. (H) Detection of gelatinase activities of MMP2 and MMP9 by gelatin zymography.
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    Image Search Results


    Activation of Wnt/β-catenin signaling enhances STB differentiation. (A) A scheme of the culture conditions. Undifferentiated macTSCs were cultured with [CHIR(+)] or without [CHIR(−)] CHIR99021 for 3 days. Some cells were induced to differentiate in the medium containing dbcAMP [CHIR(−) cAMP and CHIR(+) cAMP] for another 6 days. (B) The relative expression level of a vCTB marker, TEAD4 . (C) Phase contrast images of cells on day 9. Multinucleated STB is outlined by a red line. Scale bar = 100 μm. (D, G) The relative expression levels of STB (D) and EVT (G) markers. For C, D, and G, the mean values (±SD) of technical triplicates of biological duplicates, normalized by the expression of ACTB , are shown relative to those in CHIR(−) (arbitrarily set as 1). Different letters represent significant differences ( p < 0.05). (E) Detection of multinucleated STBs by immunofluorescence for E-cadherin, CG, and DAPI staining. Scale bar = 100 μm. (F) The mean intensity ± SD (%) of STB nuclei calculated from four randomly chosen fields of biological duplicates. Different letters represent significant differences ( p < 0.05). Note that error bars got larger as the STB ratio increased, likely because the larger the STBs formed, the sparser the distribution of STBs became so that some of the randomly selected fields contained very few STBs. (H) Detection of gelatinase activities of MMP2 and MMP9 by gelatin zymography.

    Journal: Biology of Reproduction

    Article Title: Wnt signaling activation confers a syncytiotrophoblast progenitor state on trophoblast stem cells of cynomolgus monkey †

    doi: 10.1093/biolre/ioae131

    Figure Lengend Snippet: Activation of Wnt/β-catenin signaling enhances STB differentiation. (A) A scheme of the culture conditions. Undifferentiated macTSCs were cultured with [CHIR(+)] or without [CHIR(−)] CHIR99021 for 3 days. Some cells were induced to differentiate in the medium containing dbcAMP [CHIR(−) cAMP and CHIR(+) cAMP] for another 6 days. (B) The relative expression level of a vCTB marker, TEAD4 . (C) Phase contrast images of cells on day 9. Multinucleated STB is outlined by a red line. Scale bar = 100 μm. (D, G) The relative expression levels of STB (D) and EVT (G) markers. For C, D, and G, the mean values (±SD) of technical triplicates of biological duplicates, normalized by the expression of ACTB , are shown relative to those in CHIR(−) (arbitrarily set as 1). Different letters represent significant differences ( p < 0.05). (E) Detection of multinucleated STBs by immunofluorescence for E-cadherin, CG, and DAPI staining. Scale bar = 100 μm. (F) The mean intensity ± SD (%) of STB nuclei calculated from four randomly chosen fields of biological duplicates. Different letters represent significant differences ( p < 0.05). Note that error bars got larger as the STB ratio increased, likely because the larger the STBs formed, the sparser the distribution of STBs became so that some of the randomly selected fields contained very few STBs. (H) Detection of gelatinase activities of MMP2 and MMP9 by gelatin zymography.

    Article Snippet: After nuclear counterstaining with DAPI (D523; DOJINDO, Kumamoto, Japan), the cells were mounted on glass slides with VECTASHIELD (H-1000; Vector Laboratories, Newark, CA, USA).

    Techniques: Activation Assay, Cell Culture, Expressing, Marker, Immunofluorescence, Staining, Zymography

    macTSCs acquire FSK responsiveness for STB differentiation by Wnt/β-catenin signaling activation. (A) A scheme of the macTSC culture conditions. Undifferentiated macTSCs were cultured with [CHIR(+)] or without [CHIR(−)] CHIR99021 for 3 days. Some cells were induced to differentiate in the medium containing forskolin (FSK) [CHIR(−) FSK and CHIR(+) FSK] for another 6 days. (B) Phase contrast images of cells on day 9. Multinucleated STB is indicated by a red line. Scale bar = 100 μm. (C) The relative expression levels of STB markers. The mean values (±SD) of technical triplicates of biological duplicates, normalized by the expression of ACTB , are shown relative to those of cells maintained under the CHIR(−) condition (arbitrarily set as 1). (D) Detection of multinucleated STB by immunofluorescence for E-cadherin, CG, and DAPI staining. Scale bar = 100 μm. (E) The mean intensity ± SD (%) of STB nuclei calculated from four randomly chosen fields of biological duplicates. Different letters represent significant differences ( p < 0.05). Note that error bars got larger as the STB ratio increased, likely because the larger the STBs formed, the sparser the distribution of STBs became so that some of the randomly selected fields contained very few STBs.

    Journal: Biology of Reproduction

    Article Title: Wnt signaling activation confers a syncytiotrophoblast progenitor state on trophoblast stem cells of cynomolgus monkey †

    doi: 10.1093/biolre/ioae131

    Figure Lengend Snippet: macTSCs acquire FSK responsiveness for STB differentiation by Wnt/β-catenin signaling activation. (A) A scheme of the macTSC culture conditions. Undifferentiated macTSCs were cultured with [CHIR(+)] or without [CHIR(−)] CHIR99021 for 3 days. Some cells were induced to differentiate in the medium containing forskolin (FSK) [CHIR(−) FSK and CHIR(+) FSK] for another 6 days. (B) Phase contrast images of cells on day 9. Multinucleated STB is indicated by a red line. Scale bar = 100 μm. (C) The relative expression levels of STB markers. The mean values (±SD) of technical triplicates of biological duplicates, normalized by the expression of ACTB , are shown relative to those of cells maintained under the CHIR(−) condition (arbitrarily set as 1). (D) Detection of multinucleated STB by immunofluorescence for E-cadherin, CG, and DAPI staining. Scale bar = 100 μm. (E) The mean intensity ± SD (%) of STB nuclei calculated from four randomly chosen fields of biological duplicates. Different letters represent significant differences ( p < 0.05). Note that error bars got larger as the STB ratio increased, likely because the larger the STBs formed, the sparser the distribution of STBs became so that some of the randomly selected fields contained very few STBs.

    Article Snippet: After nuclear counterstaining with DAPI (D523; DOJINDO, Kumamoto, Japan), the cells were mounted on glass slides with VECTASHIELD (H-1000; Vector Laboratories, Newark, CA, USA).

    Techniques: Activation Assay, Cell Culture, Expressing, Immunofluorescence, Staining